Finding functional sequence elements by multiple local alignment

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Finding functional sequence elements by multiple local alignment.

Algorithms that detect and align locally similar regions of biological sequences have the potential to discover a wide variety of functional motifs. Two theoretical contributions to this classic but unsolved problem are presented here: a method to determine the width of the aligned motif automatically; and a technique for calculating the statistical significance of alignments, i.e. an assessmen...

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DIALIGN: finding local similarities by multiple sequence alignment

MOTIVATION DIALIGN is a new method for pairwise as well as multiple alignment of nucleic acid and protein sequences. While standard alignment programs rely on comparing single residues and imposing gap penalties, DIALIGN constructs alignments by comparing whole segments of the sequences. No gap penalty is employed. This point of view is especially adequate if sequences are not globally related,...

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MOTIVATION We introduce a novel approach to multiple alignment that is based on an algorithm for rapidly checking whether single matches are consistent with a partial multiple alignment. This leads to a sequence annealing algorithm, which is an incremental method for building multiple sequence alignments one match at a time. Our approach improves significantly on the standard progressive alignm...

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6.8 Local Sequence Alignment

log g i,j f(j) . For large n, the resulting PAM matrices often allow one to find related proteins even when there are practically no matches in the alignment. In this case, the underlying nucleotide sequences are so diverged that their comparison usually fails to find any statistically significant similarities. For example, the similarity between the cancer-causing ν-sis oncogene and the growth...

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ژورنال

عنوان ژورنال: Nucleic Acids Research

سال: 2004

ISSN: 1362-4962

DOI: 10.1093/nar/gkh169